I, _______________________________, a duly authorised Government Veterinary Officer, after due enquiry, certify that:

1. 1) The semen was collected at a Department approved semen collection centre under the supervision of a veterinarian approved by the Department. The semen was collected and processed in accordance with the current OIE Terrestrial Animal Health Code.
   
   Approval number of semen collection centre:
   
   
2. 2) Australia is a country free from Vesicular Stomatitis, as defined by the OIE Terrestrial Animal Health Code, for at least one year prior to the collection of the semen.
   
   
3. 3) Australia is officially recognised by the OIE as a Foot and Mouth disease free country without vaccination for at least one year prior to the collection of the semen.
   
   
4. 4) Australia is a country free from Brucella abortus, as defined by the OIE Terrestrial Animal Health Code, for at least one year prior to the collection of the semen.
   
   
5. 5) The donor bulls:
   
   
1. I. had been continuously resident at the semen collection centre concerned, or at another such Department accredited semen collection centre for a period of at least one month prior to collection of the semen for export to the Republic of South Africa, and in that time had not been used for natural mating and were isolated from animals not of equivalent health status;
   
   
2. II. showed no clinical signs of infectious and contagious disease and diseases transmissible through semen during the period of residence at the semen collection centre.
   
   
3. III. have been tested for bovine tuberculosis with intradermal tuberculin tests with negative results within the twelve (12) months preceding the collection of the semen to be exported, or alternatively the country of origin is free from bovine tuberculosis;
   
   
4. IV. Trichomonas foetus: sexually immature bulls that have had no contact with females must test negative on a culture test prior to entering the semen collection centre. Other bulls must test negative on culture test OR on PCR testing on three (3) sheath washing - or preputial scraping samples collected at least seven (7) days apart, taken during the three (3) months immediately preceding date of collection;
   
   
5. V. Campylobacter foetus: sexually immature bulls that have had no contact with females must test negative on a culture test prior to entering the semen collection centre. Other bulls must test negative on culture test OR on PCR testing on three (3) sheath washing - or preputial scraping samples collected at least seven (7) days apart, taken during the twelve (12)⁽¹⁾ three (3)⁽¹⁾ months immediately preceding date of collection;
   
   
6. VI. had been tested by a microscopic agglutination test (MAT) on blood for Leptospira serovars pomona and hardjo:
   
   
1. i) with negative results (negative at 1:100) to a single test within twelve (12)⁽¹⁾ three (3)⁽¹⁾ months prior to collection of this semen,
   
   OR
   
   
2. ii) with a stabilized low titre (negative at 1:400) to two (2) tests conducted two (2) to four (4) weeks apart within three (3) months prior to collection of this semen.
   
   
3. [Note: An end or limiting titer (1:100 or greater) was run on the samples].
   
   
7. VII. originate from herds which are certified free from Johne's disease/or where no clinical, cultural or pathological evidence of Johne's disease occurred in the herd of origin for the three (3) year period before the bull entered the AI centre and each donor gave a negative result to an ELISA or CFT test for Johne’s disease prior to entering the semen collection centre;
   
   OR
   
   
8. Each donor in the twelve (12) month period prior to entering the semen collection centre, gave negative results to two (2) serological tests (ELISA or CFT) for Johne’s disease on samples collected at least six (6) months apart, and a faecal culture test.
   
   
9. VIII. were tested serologically negative for IPV/IBR with a serum neutralization test or ELISA during the twelve (12)⁽¹⁾ three (3)⁽¹⁾ months preceding collection of the semen, or an aliquot of each semen collection for this consignment was subjected to a PCR test for IBV/IBR with negative results.
   
   
10. IX. were tested serologically negative for Enzootic Leucosis on agar gel immunodiffusion (AGID) test or ELISA test during the three (3) months preceding collection of the semen.
    
    
11. X. Bovine viral diarrhoea/mucosal disease: the donor bulls were subjected to an antigen detection ELISA or virus isolation test with negative results for bovine viral diarrhoea and a serological test, prior to entry into the approved semen collection centre. Serology should be repeated at least annually with negative results. For any previously seronegative resident donor bull that has become serologically positive, an aliquot of each semen collection from that donor for this consignment must be subjected to a virus isolation test or PCR test with negative results. Animals with a negative status should remain isolated from other animals that have not tested negative.
    
    ⁽¹⁾ Delete where applicable

Note


In the case of bulls continuously resident at the Semen collection centre concerned for periods longer than three (3) months, negative results for the disease conditions mentioned above will be acceptable provided the tests were performed at the Semen collection centre during the period of residence and not more than twelve (12) months prior to the collection of the semen to exported.

1. 6) All straws are clearly and permanently marked with the identification of the donor animal and the date of collection. If a code is used for this information, its decipher must accompany the consignment.
   
   
2. 7) The semen was collected and processed under veterinary supervision in accordance with the current Terrestrial Animal Health Code.
   
   
3. 8) The semen described above was stored in approved conditions for a minimum period of thirty days immediately following collection.
   
   
4. 9) The semen is adequately, securely and hygienically packed in new or recently disinfected transport containers filled with fresh (previously unused) liquid nitrogen. The containers must be sealed under official veterinary supervision.
   
   Tank Serial No:  xxxxx
   
   Seal numbers:  xxxxx
   
   

Attachment to health certificate

ATTACHMENT TO ANIMAL HEALTH CERTIFICATE FOR EXPORT OF BOVINE AND BUFFALO SEMEN FROM AUSTRALIA TO RSA

IMPORT PERMIT No.: xxxxxxxxx; Authorisation No.: xxxxxxx