Capsicum annuum seed
| Criteria | Criteria Required? |
|---|---|
| Import Permit | No |
| Phytosanitary Certificate | Yes |
| Additional Declaration/Endorsement | Yes |
| Protocol Market | No |
- ToBRFV is not known to occur in Australia.
Sampling schemes
Sampling of seeds for testing for ToBRFV must be carried out under the following sampling schemes depending on the seed lots as referred to in the relevant table of the International Standards for Phytosanitary Measures 31 Methodologies for sampling of consignments (ISPM 31):- For seed lots less than or equal to 3,000 seeds: application of a hypergeometric sampling scheme able to identify with 95% reliability a level of presence of infected plants of 10% or above;
- For seed lots of more than 3,000 but less than 30,000 seeds: application of a sampling scheme able to identify with 95% reliability a level of presence of infected plants of 1% or above;
- For seed lots of more than 30,000 seeds: application of a sampling scheme able to identify with 95% reliability a level of presence of infected plants of 0,1 % or above.
Testing methods
One of the following testing methods must be used for the detection of ToBRFV on seeds:- ELISA (accepted only until 1 October 2020);
- Real-time PCR using the primers and probes described in the ISF protocol (2020)(Protocol developed by the International Seed Federation (International Seed Health Initiative for Vegetable Crops (ISHI-Veg));
- Real-time PCR using primers and probe of Menzel and Winter (Acta Horticulturae, in press).
If a positive result is obtained from the first test (detection test), a second testing method which is different from the one used for detection must be carried out, using the same sample to confirm the identification. If there is inconsistency between detection and identification results obtained for coated seeds, the coating of the seeds must be removed and the seeds should be retested.
- Applies to seeds of Australian origin.
- A laboratory analysis certificate must be provided as evidence that the seeds or their mother plants have been tested and found free of ToBRFV.
- Applies to seeds of Australian origin.
- A breeder's note attesting to the resistance of the seed variety to ToBRFV must be provided as evidence that the seed variety is recognised as being resistant to this virus.
- Applies to seed of non-Australian origin where testing for ToBRFV is conducted in Australia.
- A laboratory analysis certificate must be provided as evidence that the seeds have been tested and found free of ToBRFV.
- An additional declaration stating that ToBRFV is not known to occur in the country of origin, or that the mother plants of the seeds have been produced in a production site where ToBRFV is known not to occur on the basis of official inspections carried out at the appropriate time to detect ToBRFV, must be endorsed on the phytosanitary certificate issued by the country of origin.
- If the seeds remained in the original packaging and the seeds were not treated or processed in Australia, the exporter should apply for a manual re-export phytosanitary certificate (EX25) which includes an additional declaration with the exact same wording as Endorsement 5993. The EX25 must accompany the original or a certified copy of the phytosanitary certificate issued by the country of origin.
- If the consignment was opened, the seeds were treated or processed and/or re-packaged after arrival in Australia, the exporter should apply for a phytosanitary certificate for export (E16) via EXDOC, select Endorsement 5993 and include the country of origin in the additional product description field of the RFP. The original or a certified copy of the phytosanitary certificate issued by the country of origin must also accompany the consignment. To enable the additional product description field on the RFP the exporter must submit a request to the EXDOC Plant Programs via email (EXDOCPlantPrograms@awe.gov.au).
1) Tomato brown rugose fruit virus (ToBRFV) is not known to occur in Australia.
Option 3 (EXDOC Endorsement 5993)
1) The seeds have been found free through official sampling and testing procedures of Tomato brown rugose fruit virus (ToBRFV).
EU Directive 2000/29 and COMMISSION IMPLEMENTING REGULATION (EU) 2020/1191 of 11 August 2020.