Capsicum annuum seed
| Criteria | Criteria Required? |
|---|---|
| Import Permit | No |
| Phytosanitary Certificate | Yes |
| Additional Declaration/Endorsement | Yes |
| Protocol Market | No |
Sampling schemes
Sampling of seeds for testing for ToBRFV must be carried out under the following sampling schemes depending on the seed lots as referred to in the relevant table of the International Standards for Phytosanitary Measures 31 Methodologies for sampling of consignments (ISPM 31):- For seed lots less than or equal to 3,000 seeds: application of a hypergeometric sampling scheme able to identify with 95% reliability a level of presence of infected plants of 10% or above;
- For seed lots of more than 3,000 but less than 30,000 seeds: application of a sampling scheme able to identify with 95% reliability a level of presence of infected plants of 1% or above;
- For seed lots of more than 30,000 seeds: application of a sampling scheme able to identify with 95% reliability a level of presence of infected plants of 0,1 % or above.
Testing methods
One of the following testing methods must be used for the detection of ToBRFV on seeds:
- Real-time PCR using the primers and probes described in the ISF protocol (2020)(Protocol developed by the International Seed Federation (International Seed Health Initiative for Vegetable Crops (ISHI-Veg));
- Real-time PCR using primers and probe of Menzel and Winter (Acta Horticulturae, in press).
If a positive result is obtained from the first test (detection test), a second testing method which is different from the one used for detection must be carried out, using the same sample to confirm the identification. If there is inconsistency between detection and identification results obtained for coated seeds, the coating of the seeds must be removed and the seeds should be retested.
- A laboratory analysis certificate must be provided as evidence that the seeds or their mother plants have been tested and found free of ToBRFV.
- A breeder's note attesting to the resistance of the seed variety to ToBRFV must be provided as evidence that the seed variety is recognised as being resistant to this virus.
1) Tomato brown rugose fruit virus (ToBRFV) is not known to occur in Australia.
EU Directive 2000/29 and COMMISSION IMPLEMENTING REGULATION (EU) 2020/1191 of 11 August 2020.