Disease tested for |
Timing |
Method of testing |
EU requirements re method of testing |
Bovine tuberculosis |
On-farm- within the 28 day period before Quarantine; AND | Intradermal tuberculin test - cervical site | Dir. 64/432/EEC details the specifications for the purified protein derivative. |
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On-centre- at least once yearly | Intradermal tuberculin test - cervical site |
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Bovine brucellosis |
On-farm- within the 28 day period before Quarantine; AND | ELISA, CFT, Rose Bengal Test, SAT, FPA | Laid down in Annex C to Dir. 64/432/EEC. |
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Quarantine- at least 21 days after admittance; AND | ELISA, CFT, Rose Bengal Test, SAT, FPA |
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On-centre- at least once yearly | ELISA, CFT, Rose Bengal Test, SAT, FPA |
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Enzootic bovine leucosis |
On-farm- within the 28 day period before Quarantine; AND | AGID, ELISA | Laid down in Annex D Chapter II to Dir. 64/432/EEC. |
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On-centre- at least once yearly | AGID, ELISA |
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IBR/IPV |
On-farm- within the 28 day period before Quarantine; AND | A serological test - whole virus | "...a serological test (whole virus) on a blood sample...as defined in Article 2.3.5.3 of the International Animal Health Code;" |
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Quarantine- at least 21 days after admittance; AND= | =A serological test - whole virus= |
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On-centre- at least once yearly | A serological test - whole virus |
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BVD/MD |
On-farm- within the 28 day period before Quarantine; AND | Virus isolation or virus antigen | "...a virus isolation test or a test for virus antigen..." |
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On-farm- within the 28 day period before Quarantine; AND | Serological test *Positive titres are allowed | "...a serological test to determine the presence or absence of antibodies..." |
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Quarantine- at least 21 days after admittance; AND | Virus isolation or virus antigen | "...a virus isolation test or a test for virus antigen..." |
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Quarantine- at least 21 days after admittance; AND | Serological test *Positive titres are allowed, but only if positive titre during on-farm testing i.e. no seroconversion allowed | "...a serological test to determine the presence or absence of antibodies..." |
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On-centre- at least once yearly | Serological test * Applied only to resident bulls that were serologically negative at admittance. No seroconversion allowed. | "...a serological test to determine the presence or absence of antibodies..." |
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On-centre- prior to first dispatch of semen | Virus isolation on first semen sample collected - must have a negative result * Applied only to resident bulls that were serologically positive at admittance. | No further guidance given - consistent with OIE manual |
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Campylobacter fetusssp.
venerealis |
Quarantine- at least 7 days after admittance; AND | Preputial specimen - isolation and identification of agent. Required either once or 3 times at weekly intervals. | No further guidance given - consistent with OIE manual |
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On-centre- at least once yearly | Preputial specimen - isolation and identification of agent. | No further guidance given - consistent with OIE manual |
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Trichomonas foetus |
Quarantine- at least 7 days after admittance; AND | Preputial specimen - isolation and identification of agent. Required either once or 3 times at weekly intervals. | No further guidance given - consistent with OIE manual |
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On-centre- at least once yearly | Preputial specimen - isolation and identification of agent. | No further guidance given - consistent with OIE manual |
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EHD (all serotypes present in Australia - 1, 2, 5, 6, 7 and 8) |
On-centre. Test timing must apply to the collection for each exported consignment. Either: | | "Standards for EHD virus diagnostic tests are described in the bluetongue chapter of the Manual of Diagnostic Tests and Vaccines for Terrestrial Animals." |
• Two serological tests not more than 12 months apart - one prior to collection and one no less than 21 days post collection. OR | CFT, AGID, cELISA |
| • Serological tests throughout the collection period at intervals of no more than 60 days, and between 21 and 60 days post final collection. OR | CFT, AGID, cELISA |
| • Agent identification tests at commencement and conclusion of collection period, as well as at least every 7 days (virus isolation test) or every 28 days (PCR) during the collection period | Virus isolation or PCR |
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Bluetongue | EITHER: | | |
| • Animals were kept in a bluetongue virus-free country or zone for at least 60 days prior to, and during, collection of the semen for the consignment; OR | No BT testing required | Consistent with the OIE Manual |
| • Animals were kept in an establishment protected from Culicoides for at least 60 days prior to, and during collection of the semen for the consignment; OR | No BT testing required |
| • Serological tests for antibody at least every 60 days throughout collection, and between 21 and 60 days after final collection for the consignment; OR | cELISA or AGID |
| • Agent identification test at commencement and conclusion of semen collection for the consignment AND during the collection period, either virus isolation every 7 days or PCR every 28 days. | Virus isolation or PCR as per schedule |
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