1. AQIS can commence the quarantine and inspection procedure of
animals to obtain genetic material for export to china after
receiving the relevant import permit from the Chinese
authority.

2. The Chinese will send veterinarians to the embryo transfer
centres of origin and related laboratories to cooperate with
the Australian veterinarians in carrying out inspections and
quarantine requirements.

3. The embryo transfer centre must be registered by the
Department of Agriculture Fisheries and Forestry - Australian
Quarantine and Inspection Service.

4. The males donating the semen must be from an Australian
approved artificial breeding or embryo transfer centre and
meet the disease freedom and isolation, testing and treatment
requirements specified for the donor females.

Australia to be free from -
.Foot and mouth disease
.Peste des petits ruminants
.Sheep pox
.Goat pox
.Wesselsbron disease
.Nairobi sheep disease
.Contagious agalactia

The premises of origin for the donor females are to be officially confirmed free from-
.Scrapie for 5 years
.Tuberculosis for 3 years
.Maedi/visna for 5 years
.Caprine retrovirus infection for 5 years
.Brucellosis for 3 years
.Paratuberculosis (Johne's disease) for 2 years
.Chlamyaia disease clinically for 2 years
.Border disease clinically for 2 years
.Mucosal disease clinically for 2 years
.Campylobacter fetus clinically for 2 years
.Bluetongue clinically for 3 years within a 50km radius

During the period from 30 days prior to semen insemination to 7 days after collection of embryos, the donor animals shall be held at the embryo transfer centre in isolation from other animals.

During the period mentioned above the animals will be individually clinically examined and tested for the following diseases with negative results-

.Brucella ovis and abortus 30 days prior to insemination by complement
fixation test (CFT) negative is less than 10iu/ml

.Paratuberculosis (Johne's disease) by intradermal Johnin PPD
test at the cervical site negative being a reaction of not
more than 2mm at the site of injection and complement
fixation test (CFT) negative is less than 50% fixation at
1:10

.Bluetongue:
-30 days prior to insemination agar gel immunodiffusion
test (AGID), complement fixation test (CFT) negative
being less than 50% fixation at 1:5, and for animals
coming from north of 28 degrees in Western Australia;
other than the following Pasture Protection Boards in New
South Wales:- Broken Hill, Wentworth, Balranald,
Moulamein, Deniliquin, Jerilderie, Corowa, Urana, Albury,
Hume and Bombala; Queensland; and the Northern Territory
chick embryo blood inoculation (3 passages for virus
examination
-30 days after embryo collection by agar gel
immunodiffusion test (AGID)
and
-from the semen collected take 3 straws for chick embryo
semen inoculation (3 passages for virus examination) with
only males whose semen gives negative results being used
for insemination of the donors

.Mucosal disease and Border disease 30 days prior to
insemination and at the time of collection by examination for
virus in blood serum with 2 passages in tissue culture
(cultures to be checked for virus by immunofluorescence test)
.Chlamydia 30 days prior to insemination complement fixation
test (CFT) negative is less than 50% fixation at 1:4

.Akabane:
-30 days prior to insemination by serum neutralisation
test (SNT) negative at 1:4
-30 days after collection of embryos agar gel
immunodiffusion test (AGID)

.Retrovirus infection:
-30 days prior to insemination by agar gel immunodiffusion
test (AGID)
-30 days after collection by agar gel immunodiffusion test
(AGID)

The collection and handling of the embryos will be done under the supervision of an Authorised Australian Veterinarian.

The membrane of the embryos will be in sound condition and the embryos will be flushed (washed) 10 times according to the methods suggested by the International Embryo Transfer Society.

The embryos will be placed immediately after collection into vials which will be sealed, numbered, and identified as to donor dam and sire and recorded with test results, dates and places of insemination and collection of embryos.

The embryos will be stored at the centre under the supervision of the authorised veterinarian and allowed to be shipped once negative results are received at the end of the quarantine period following embryo collection.

Obtainable from:

State General Administration of the People's Republic of China
For Quality Supervision, Inspection and Quarantine (AQSIQ)
9 Madiandonglu
Haidian District
Beijing 100088 CHINA

SEPTEMBER 1989