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Live animals

Cattle embryos

Country: Chile

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Pre-export preparations

Semen used to fertilise the ova must be derived from and conform to the current import conditions for bovine semen to Chile.

Special conditions

Please note that all facilities that wish to collect embryos for
export to Chile must be inspected and approved Chilean (SAG) government
veterinary officers. These visits are conducted at the expense of the
party wishing to obtain SAG approval, and can take considerable time to
organise. Embryos are not permitted for importation if collected prior
to the centre obtaining SAG approval (and this applies also to semen used
in preparation of the embryo consignment). You are strongly advised to
take this into consideration when planning a consignment. Please contact
AQIS for further information.

OIE LIST A DISEASES (BOVINE) ARE DEFINED AS:
Bluetongue, Contagious Bovine Pleuropneumonia, Foot and Mouth Disease, Lumpy Skin Disease, Rift Valley Fever, Rinderpest and Vesicular Stomatitis

OIE LIST B DISEASES (BOVINE) ARE DEFINED AS:
Anaplasmosis, Anthrax, Babesiosis, Bovine Spongiform Encephalopathy, Bovine Tuberculosis, Brucellosis, Dermatophilosis, Echinococcosis / Hydatidosis, Enzootic Bovine Leukosis, Genital Campylobacteriosis, Heartwater, Hemorrhagic Septicemia, Infectious Bovine Rhinotracheitis / Infectious Pustular Vulvovaginitis, Leptospirosis, Malignant Catarrhal Fever, Nairobi Sheep Disease, Paratuberculosis, Pseudorabies, Q-Fever, Rabies, Screwworm, Theileriosis, Trichomoniasis, Trypanosomiasis, Tuberculosis, Viral Hemorrhagic Septicemia).

Product processing

1. The embryos must be selected and processed by a collection team which is
officially approved and undergoes official supervision by veterinary
authorities at least twice a year.
2. The selected group has not operated during the procedure in areas
infected with the diseases described in List A of the OIE.
3. The processes of securing, processing, storage and transportation
the embryos must be carried out in accordance with the procedures
approved by IETS and OIE.
4. The embryos must be washed in groups of 10 or less, with 10 changes
of medium. A fresh sterilized micro-tube must be used to transfer the
embryos between washes and each one of them constituted a dilution
of at least 1/100 of the previous washing medium.
5. Only the embryos of the same donor may be washed together.
6. The embryos must further undergo treatment with trypsin in accordance
with the IETS procedural manual.
7. Prior to any micromanipulation or freezing procedure, the entire
surface of the embryos must be examined with a microscope of a minimum
magnification of 50x in order to guarantee that the zona pellucida is intact
and free of any adhesive material.
8. The embryos must be placed in straws or ampoules, identified with the breed
and identification of the two donors, the collection date, and the
identification of the embryo collection team.

Health certification

1. The exporting country must be declared free of foot-and-mouth disease,
bovine fever (Rhinderpest), contagious bovine pleuropneumonia, contagious
nodular dermatosis (lumpy skin disease), BSE, vesicular stomatitis and Rift
Valley fever disease before the International Office of Epizooties.

2. The semen used for the production of the embryos must be derived from semen
batches which conform to Chilean import conditions for semen from Australia.

3. The donor must be born in, or has remained in the country for at least
6 uninterrupted months prior to the collection of the embryos destined
for Chile.

3. The donor must be inspected on the day of collection, and found to be
free of any evidence of OIE List A and B diseases known to be transmitted
by embryo transfer.

4. The premises of origin of the donor must be officially free of brucellosis
and tuberculosis.

5. No clinical evidence of OIE List A and B diseases known to be transmitted
by embyro transfer must be present on the premises of origin of the donor.

Transport requirements

1. The embryos must be stored in a safe place approved for these means by
the appropriate health authority, under the supervision of the veterinary
surgeon specialized in embryo transference accredited by the health
authority to export embryos, who is responsible for the completion
of all operations and certifications taken into account in these
health requirements.
2. While the embryos to be exported to Chile are manipulated until after
storage, there must be no processing of embryos of a state of health
different to these health requirements.
3. The shipping container must be new or must have been cleaned and
disinfected in the appropriate way, and only virgin liquid nitrogen
must be used.

Permit requirements

Written advice from SAG of approval for importation of the consignment may be required at export by AQIS.

Health certificate

HEALTH CERTIFICATE

IDENTIFICATION

1. Place where the embryos were obtained
2. Name and management of the embryo transference agency carrying out
the embryo collection
3. Semen identification: identification of the donor and of the
artificial insemination centre
4. Number of embryos in the batch
5. Date of the embryo collection
6. Date of the semen collection used in the fertilization of these
embryos
7. The blood group of both parental donors and certification that, to date,
carriers of deleterious genes have not been identified.

I, ____________________, the undersigned, a duly authorized AQIS
Veterinary officer certify that:

THE COUNTRY
1. Australia is free according to the OIE of the following diseases:
- Foot and mouth disease
- Bovine fever (Rhinderpest)
- Contagious bovine pleuropneumonia
- Contagious nodular dermatosis (lumpy skin disease)
- BSE
- Vesicular stomatitis
- Rift valley disease (Rift Valley fever)

THE SEMEN

2. The semen used for the production of the embryos conforms to the
Chilean import conditions for semen from Australia.

THE DONOR

3.1 The donor was born in or has remained in the country for at least
6 uninterrupted months prior to the embryo collection destined for Chile.

3.2 The donor was inspected on the day of collection, and was found to be
free of any evidence of OIE List A and B diseases known to be transmitted
by embryo transfer.

3.3 The premises of origin of the donor is officially free of brucellosis
and tuberculosis.

3.4 No clinical evidence has been found in the premises of origin of
OIE Lists A and B diseases known to be transmitted by embryo transfer.

THE EMBRYOS

4.1 The embryos were selected and processed by the collection team,
identified in this certificate, which are officially approved
and who undergo official supervision by veterinary authorities at
least twice a year.

4.2 The selected group has not operated during the procedure in areas
infected with the diseases described in List A of the OIE.

4.3 The processes of securing, processing, storage and transportation
of the embryos were carried out in accordance with the procedures
approved by IETS and OIE.

4.4 The embryos were washed in groups of 10 or less, with 10 changes
of medium. A fresh sterilized micro-tube was used to transfer the
embryos between washes and each one of them constituted a dilution
of at least 1/100 of the previous washing medium.

4.5 Only the embryos of the same donor were washed together.

4.6 The embryos underwent treatment with trypsin in accordance with the
IETS procedural manual.

4.7 Prior to any micromanipulation or freezing procedure, the entire
surfaces of the embryos were examined with a microscope of a
magnification of 50x in order to guarantee that the zona pellucida
was intact and free of any adhesive material.

4.8 The straws containing the embryos are marked with the breed and
identification of the two donors, the collection date and the
identification of the embryo collection team.

STORAGE AND TRANSPORTATION

5.1 The embryos were stored in a safe place approved for these means
by the appropriate health authority, under the supervision of the
veterinary surgeon specialized in embryo transference accredited
by AQIS to export embryos, who is responsible for the completion
of all operations and certifications taken into account in these
health requirements.

5.2 While the embryos to be exported to Chile were manipulated and
until the embryos were stored, there was no processing of other
embryos of a state of health different to these health requirements.

5.3 The shipping container is new or has been cleaned and disinfected
in the appropriate way, and only virgin liquid nitrogen has been used.

Protocol last negotiated

FEBRUARY 2003

Latest updates

4608|09/10/2020|20211119|5632|NoSC
Last updated: 3/07/2022 11:29 PMCreated: 15/01/2015 12:43 PM